Description
The X1-STORM is a multi-modal system that boasts super-resolution fluorescence microscopy and live-cell spinning-disk confocal microscopy by combining N-STORM capabilities and CSU-X1 hardware on one microscope body, united by MetaMorph software.
Technical Specifications
Objective lens: 10x, 60x (oil), 100x (oil)
Filters & Dichroics: DAPI, GFP, mCherry, CY5, TIRF 488/561, TIRF Quad, NSTORM Quad
Laser lines: 405 nm, 488 nm/515 nm, 561 nm, 647 nm
Camera: Hamamatsu ORCA-Quest, Hamamatsu ORCA-FusionBT
Stage: Nikon TI-S-ER Motorized Stage
Software: NIS-Elements AR, MetaMorph
Model
NSTORM; CSU-X1
Manufacturer
Nikon;
Yokogawa
Application
• 2D-STORM
• 3D-STORM
• TIRF imaging
• Spinning-disk confocal microscopy
• Live cell imaging
Special Remarks
The system enables super-resolution imaging at tenfold the resolution of conventional light microscopes and enables visualization of molecular interactions and organizations at the nanoscopic scale after careful optimization of sample preparation, image acquisition and image reconstructions. Additionally, large FOV TIRF can be achieved due to the ORCA-Quest qCMOS. As an addition modality, live cell confocal microscopy can be carried out by the CSU-X1 module.
Location
Mechanobiology Institute, Microscopy Core (MBI-SIMBA)
Temasek Laboratories @ NUS
Level 9 Microscopy Room 7
5A Engineering Drive 1, Singapore 117411
Booking Information
Charges: N/A
Service or Self-operated: Self-operated
Website for booking: https://ppms.asia/singascope/login/
Key Contact(s)
Melvino Sin
melvino@nus.edu.sg
Anne Beghin
mbianne@nus.edu.sg
Characterisation | Analysis | Imaging | Life Science | MBI | Equipment