Description
For department/faculty use only.
Multiphoton excitation occurs when two (or more) photons, whose summed energy is sufficient to excite a fluorophore, arrive simultaneously. The use of near infrared light to excite a fluorophore in the visible range allows multiphoton microscopy to image deeper within tissue than confocal microscopy. Additionally, since the signal is spatially confined to the focal plane of the objective, it eliminates the necessity for a confocal pinhole to generate optically sectioned images, which greatly improves detection sensitivity. The spatial confinement of signal generation also reduces overall photobleaching. These advantages make multiphoton microscopy the ideal choice for imaging deep within live tissue over long periods of time.
Technical Specifications
2 photons microscope
Two independent laser-scanning units
Resonant-galvo scan optics with 680 to 1600 nm achromatic performance and anti-reflection coating
Galvo-galvo scan optics with 400 to 1100 nm achromatic performance and anti-reflection coating
Binocular lens available
Wide-field epi-fluorescence imaging
The 8kHz resonant scanner and galvo mirrors for X scanning, and flexible scanning modes (square, rectangular and curved/straight line scanning).
Capability of interchange two objectives
Fine/coarse Z-focus movement, and XY travel
Model
multiphoton
Manufacturer
Thorlabs
Application
2 photons microscope
Special Remarks
Only open to Duke-NUS users
Location
Duke-NUS Animal Facility
Level 1
Duke-NUS Medical School
8 College Rd, Singapore 169857
Booking Information
Charges: contact the person in charge
Service or Self-operated: Self-operated after training
Website for booking: https://inetapps.duke-nus.edu.sg/ebooking/
Key Contact(s)
Shawn Je
shawn.je@duke-nus.edu.sg
Biology | Biomedical Science | Medicine | DUKE NUS | Imaging | Equipment