SAFe360 STORM

Description
The SAFe 360 STORM is a highly motorised laser scanning microscope for multiple 3D SMLM modalities: PALM, dSTORM , PAINT, Single Particle Tracking.

Technical Specifications
Objective lens: 100x (oil)
Filters: DAPI, GFP, RFP, Cy5
Laser lines: 405 nm, 488 nm, 561 nm, 640 nm
Detector: 2x ORCA-FusionBT
Microscope: Olympus IX83
Software: Neo LiveImage, Neo Analysis

Model
SAFe 360

Manufacturer
Abbelight

Application
• PALM
• dSTORM
• PAINT
• Single particle tracking
• 3D SMLM imaging
• Spectral far-red demixing
• Simultaneous multi-color imaging

Special Remarks
The SAFe illumination device is an add-on module that can be easily integrated into any inverted microscope with a camera port. It transforms the microscope into a multimodal platform with advanced SMLM features. The module has the unique excitation system, which is based on Adaptable Scanning for Tunable Excitation Regions (ASTER) technology. ASTER generates homogenous illumination in TIRF, HiLo, and Epi modes while performing SMLM modalities, such as PALM, STORM, or PAINT with a localization precision reaching 10–15 nm in 3D, on a FOV of 150 × 150 μm2.

Location
Mechanobiology Institute, Microscopy Core (MBI-SIMBA)
Temasek Laboratories @ NUS
Level 9 Microscopy Room 2
5A Engineering Drive 1, Singapore 117411

Booking Information
Charges: N/A
Service or Self-operated: Self-operated
Website for booking: https://ppms.asia/singascope/login/

Key Contact(s)
Melvino Sin
melvino@nus.edu.sg

Anne Beghin
mbianne@nus.edu.sg

Characterisation | Analysis | Imaging | Life Science | MBI | Equipment