Description
The MAGPIX® combines the fluidics subsystem, mechanical subsystem, an electronic subsystem, and an optical sub- system with magnetic microspheres and complex computer analysis to perform multiplex assays. The MAGPIX system operates by using magnetic beads (microspheres) that are coated with a reagent specific to a particular bioassay, enabling the capture and detection of specific analytes from a sample. The sample mixture is aspirated by the sample probe and conveyed via drive fluid into the camera chamber, where the microspheres are pulled down into a monolayer by the magnet, immobilized, and imaged. Within the chamber, microspheres are exposed to a red LED and a green LED, which excite both the internal dyes that identify each bead’s color signature and the reporter fluorescence from the surface of the microspheres. The red LED is responsible for classifying the microspheres. The CL1 and CL2 filters function to categorize the microspheres based on color signature and place them properly on the bead map as well as throw out any doublets that may exist. The green LED with the RP1 filter excites the reporter fluorescence, which identifies the quantity of analyte captured for each bead region. The microspheres are then flushed to the waste fluid bottle, clearing room for the next sample.
Technical Specifications
Analyse up to 50 biomarkers in a sample with minimum 25 µL simultaneously
High-throughput
Model
Luminex® MAGPIX®
Manufacturer
MILLIPLEX®
Application
• Drug discovery
• Biomarker quantification
Special Remarks
N/A
Location
Drug Discovery & Optimization Platform
NUS Medicine MD3-05-02
16 Medical Dr, Singapore 117600
Booking Information
Charges: https://medicine.nus.edu.sg/core-facilities/drug-discovery-and-optimization-platform-ddop/charges/
Service or Self-operated: Service and self-operated
Website for booking: https://medicine.nus.edu.sg/core-facilities/drug-discovery-and-optimization-platform-ddop/faq/
Key Contact(s)
Winston Liao Wupeng
ddop@nus.edu.sg
Biology | Characterisation | Analysis | SOM | Equipment