Description
The iLAS2 TIRF is a total internal reflection fluorescence microscope for imaging close to the coverslip surface in multiple dimensions (xyt in multiple channels).
Technical Specifications
Objective lens: 10x, 20x, 60x Oil, 100x Oil
Filters: FITC, TRITC
Laser lines: 405 nm, 491 nm, 532 nm, 561 nm, 642 nm
Dichroic: Quadband dichroic and bandpass filter (single camera), T585LP dichroic with 525/45 & 609/54 (dual camera)
Cameras: 2x Hamamatsu Fusion BT (6.5 µm/pixel)
Stage: Prior Motorised XY Stage
Software: MetaMorph, Modular GATACA
Model
iLas 2
Manufacturer
Gataca Systems
Application
• TIRF imaging
• Fluorescence recovery after photobleaching (FRAP)
Special Remarks
The iLas2 system is a unique ring TIRF system with azimuthal averaging that provides illumination uniformity for TIRF/wide-field imaging. The fast response time of the motorized TIRF angle and dual camera setup allow for simultaneous multi-wavelength TIRF imaging with penetration depth adaptation. It has a dual laser illuminator for TIRF imaging and FRAP/FLIP/PA applications.
Location
Mechanobiology Institute, Microscopy Core (MBI-SIMBA)
Temasek Laboratories @ NUS
Level 10 Microscopy Room 8
5A Engineering Drive 1, Singapore 117411
Booking Information
Charges: N/A
Service or Self-operated: Self-operated
Website for booking: https://ppms.asia/singascope/login/
Key Contact(s)
Jasmine Chin
mbicflj@nus.edu.sg
Anne Beghin
mbianne@nus.edu.sg
Characterisation | Analysis | Imaging | Life Science | MBI | Equipment