Description
This microscope performs epifluorescence (also known as wide-field), rather than confocal fluorescence microscopy.
In epifluorescence microscopy, the entire specimen is flooded with light allowing visualisation of the entire depth of the sample. Confocal microscopes (such as the LSM700 and LSM780) use point illumination and a pinhole to eliminate out-of-focus signal and provide you with a thinner focal plane.
This microscope is great for imaging immunohistochemistry samples, and also for imaging living cells in culture over extended time periods. The software allows one to image several distinct coordinates (e.g. different wells) at set time periods (e.g. every 30 minutes) on cells in culture. The temperature, CO2 and humidity controlled chamber enables live cell experiments to be performed over extended time periods, e.g. viral plaque formation assays, scratch assays etc. It is equipped with low-light digital image capture and is fully automated.
Technical Specifications
Applications:
• High resolution phase-contrast, DIC and epifluorescence imaging
• Time lapse with Multi-position capability
• Imaging and analysis of live cells or fixed specimen
Model
AxioObserver Z1
Manufacturer
Zeiss
Application
Confocal microscopy
Special Remarks
N/A
Location
Confocal Microscopy Core
NUS Medicine MD1-B1-03
12 Science Drive 2, Singapore 117549
Booking Information
Charges: https://medicine.nus.edu.sg/core-facilities/confocal-microscopy/service-and-charges/
Service or Self-operated: Service and self-operated
Website for booking: https://medicine.nus.edu.sg/core-facilities/confocal-microscopy/contact-us/
Key Contact(s)
Lee Shu Ying
medleesy@nus.edu.sg
Biology | Imaging | SOM | Equipment