Confocal Microscope – Leica SP8

Description
True spectral imaging with AOBS (acousto-optical programmable beam splitter) provides highest flexibility switching in microseconds prolong sample viability with low power lasers. Tandem scanner with resonance scanner allows ultrafast 420 frames/sec (at 512 x 16 scan format) that is up to 50% faster image acquisition. Highly sensitive HyD detector allows a reduction of light dosage delivered to the cells, reducing photo-toxicity, and for detection of very low signals. The 3D rendering and imaging processing tools provide superb 3D reconstruction and high-quality images for analysis and publication.

Technical Specifications
Objectives:
• Leica HC plan apochromat 10X/0.4, & 20X/0.75
• HCX plan apochromat 40X/1.3 oil
• HC plan apochromat 63X/1.4 oil

Lasers:
• L1 coherent diode 50 mW 405 nm
• Diode 20 mW 488 nm
• Diode 20 mW 552 nm
• Diode 30 mW 638 nm

Filter sets: DAPI, FITC & TRITC
Detectors: 2HyD sensitive detectors & 1 PMT detector

Model
SP8

Manufacturer
Leica

Application
Laser scanning confocal imaging for advanced multi-colour semi-super resolution 3D imaging system for fixed cells/tissues

Special Remarks
N/A

Location
Cancer Science Institute, Microscopy & Multiplex Assays Core
NUS Medicine MD6 Centre for Translational Medicine
13 South, Confocal Microscope Room
14 Medical Dr, Singapore 117599

Booking Information
Charges: N/A
Service or Self-operated: Self-operated
Website for booking: https://csi.nus.edu.sg/staff-access/

Key Contact(s)
Dedy Sandikin
dedys@nus.edu.sg