Description
The INST1000A PhenoCycler & PhenoImager Fusion is a cutting-edge imaging system that combines bright field and fluorescence imaging capabilities for comprehensive tissue analysis. This system is an advanced automated system designed for high-plex single-cell spatial proteomics. It enables rapid, multiplexed imaging of tissue samples, allowing researchers to visualize and quantify numerous biomarkers simultaneously. The PhenoCycler offers high-resolution imaging, fast cycle times, and robust data integration capabilities, making it ideal for applications in cancer research, immunology, and neuroscience. It streamlines complex tissue analysis workflows, enhancing the accuracy and efficiency of high-throughput studies.
Technical Specifications
Multispectral range: 440 to 780 nm
Detection method: Brightfield and Fluorescence
40x throughput: Fluorescence (1.5 x 1.5 cm): 18 min
40x throughput: Brightfield (1.5 x 1.5 cm): 9 min
Automation: Touchless, with walk-away image acquisition
Slide capacity: Up to 4 slide
Multiplexing capability: Separates up to 7 colors
Imageable tissue thickness: ≤ 10 um
Compatible coverslip dimensions: 22 mm x 22 mm
Biomarker capacity: Supports up to 35 cycles
Speed: Fluorescence 45 min
Resolution: up to 0.25 um/pixel (40x)
Throughput: 1 slide
Supports up to 100 biomarkers depending on barcode compatibility
The CODEX software suite includes modules for Integration and synchronization with microscopes, image processing and data storage, and visualization and analysis of images and cell phenotypes.
Model
PCF 2.0
Manufacturer
Akoya Biosciences, United States
Application
Bench-top fluidics with automated image acquisition through automated cyclical reporter-dye delivery, supporting the detection of over 40 biomarkers with spatially resolved, ultra-highplex data and morphological context. The Phenoimager integrates seamlessly with PhenoCycler (formerly CODEX) to provide high-plex solutions, while inForm® and phenoptrReports tissue analysis software offer configurable projects for biomarker quantification and spatial analysis. This approach also maintains tissue integrity by combining CODEX chemistry with advanced fluidics to preserve tissue architecture, allowing for reuse.
Special Remarks
Spatial imaging systems are placed in Spatial Analytics Lab at level 12 N as part of deep spatial imaging program in CSI.
Location
Cancer Science Institute, Microscopy & Multiplex Assays Core
NUS Medicine MD6 Centre for Translational Medicine
12-01A North, Spatial Analytics Lab
14 Medical Dr, Singapore 117599
Booking Information
Charges: N/A
Service or Self-operated: Service required
Website for booking: https://csi.nus.edu.sg/staff-access/
Key Contact(s)
Peng Yanfen
csipy@nus.edu.sg
Biology | CSI | Imaging | Equipment