Description
The Electrophysiology Core@NUS Medicine was established in 2018. Over the years the core has accumulated extensive experience in (1) characterizing disease associated ion channel mutations (2) screening of novel modulators of ion channels (3) characterizing primary or hIPSC-derived neurons or cardiomyocytes. (4) performing patch clamp recording from acutely isolated brain slice (5) recording end-plate potential in neuromuscular junction from acutely isolated phrenic nerve-diaphragm preparation (6) performing whole endolysosomal recording. The core have worked closely with researchers in NUS/NUHS and beyond NUS, it has been engaged by DSO National Laboratories (DSO), A*STAR, NTU, SGH and various industrial partners. The core has supported high-impact publications in journals such as Nature Communications, Hypertension, PNAS, Cell Research and etc.
Technical Specifications
Nikon Ti inverted fluorescent microscope
Nikon upright patch clamp microscope
Manipulator MP-285/ MPC-325-2
MultiClamp 700B Digidata 1550 Low-Noise Data Acquisition System (Axon instruments)
Vapor pressure osmometer 5520
Flaming/brown micropipette puller model P-97
Micro forge MF-900
Single stage glass microelectrode puller MODEL PP-830
SH27B In-line solution heaters and CC-28 Thermistor
A365 Stimulus Isolator
Olympus IX81 microscope with Olympus DP71 camera
NEPA21 Electroporator
Model
NA
Manufacturer
NA
Application
Patch-clamp electrophysiology
Special Remarks
N/A
Location
Electrophysiology Core
NUS Science S9 Level 11
4 Science Drive 2, Singapore 117544
Booking Information
Charges: https://medicine.nus.edu.sg/core-facilities/electrophysiology/service-and-charges/
Service or Self-operated: Service required
Website for booking: https://medicine.nus.edu.sg/core-facilities/electrophysiology/
Key Contact(s)
Huang Hua
phshhua@nus.edu.sg
Biology | Cell and Tissue Work | SOM | Equipment