Description
The Luminex Instrument System sets the standard for multiplexing, offering the ability to perform up to 100 different tests in a single reaction volume on a flow cytometry–based platform. Using xMAP bead-based technology, Luminex multiplex assays can simultaneously detect and quantify up to 80 secreted proteins in a single well using kits from various manufacturers. Their extensive library includes cytokines, chemokines, growth factors, and other proteins across multiple species such as human, mouse, rat, non-human primate, canine, and porcine, providing comprehensive insights for diverse biological research. Additionally, this multiplex technology is adaptable for gene expression analysis. When combined with branched DNA (bDNA) technology, it can measure up to 80 gene targets with specific multiplex assays. This capability enables multi-omics studies on a single validated platform, as supported by numerous publications. The Luminex system offers accuracy and sensitivity comparable to traditional methods like ELISA and qPCR but with significantly greater efficiency and throughput.
Technical Specifications
• Detection technology: Dual laser-based flow cytometry
• Multiplexing capability: Up to 100 distinct bead regions
• Assay capacity: Supports single-plex to high-plex assays (up to 80 analytes)
• Plate compatibility: Compatible with standard 96-well and 384-well plates
• Software: xPONENT™ Software for data acquisition and analysis
• Integration: Compatible with various data analysis platforms and export formats
• Assay kits: Compatible with a wide range of Luminex assay kits and third-party kits
• Sample species: human, mouse, rat, non-human primate, canine, porcine and others
• Sample type: blood plasma, blood serum, urine, cerebrospinal fluid, synovial fluid, cell culture supernatant / conditioned cell culture medium, cell lysates, cell tissue lysates and other biological samples
• Sample volume: Requires minimal sample volume (typically 25-100 µL per well)
Model
Luminex 200
Manufacturer
Luminex
Application
High throughput quantitative proteomics or transcriptomic by multiplexing
Special Remarks
Location
Biochemistry
NUS Science S9
4 Science Drive 2, Singapore 117544
Booking Information
Charges: Contact Dr Marek Kukumberg at obgmar@nus.edu.sg
Service or Self-operated: Service required
Website for booking: https://rb.gy/tqfjc
Key Contact(s)
Marek Kukumberg
marek.kukumberg@nus.edu.sg
Sabrina Adam
med.sabrina@nus.edu.sg
Characterisation | Analysis | Cell and Tissue Work | Equipment